Purification Process for Monoclonal Antibodies without Protein A Chromatography
Auf einen Blick
- Projektleiter/in : Prof. Dr. Christiane Zaborosch
- Projektteam : Miriam Iten, Iwo König
- Projektstatus : abgeschlossen
- Drittmittelgeber : Dritte
- Projektpartner : Merck KGaA
Beschreibung
Monoclonal antibodies are usually purified using protein A media
for capture. Protein A media are expensive and leaching of the
toxic protein A is a disadvantage. Therefore, a purification
process avoiding protein A chromatography is an attractive
alternative. The goal of the project was to analyse three different
chromatography types (cation exchange, anion exchange, HIC) for
their ability to purify mAb from cell culture supernatant with a
special focus on depletion of host cell proteins and DNA. Different
ion exchange and hydrophobic interaction media (all Merck KGaA)
were used for purification of a mAb from CHO cell culture
supernatant. For each chromatography medium, optimal purification
conditions were evaluated and the depletion of host cell proteins
(HCP), ssDNA and dsDNA were analysed.
The experiments demonstrate that all three chromatography types are
capable of significantly reducing the host cell protein and DNA
content. The results also show that a purification process
comprising a sequence of these three chromatography steps can
become a suitable alternative to a purification process with an
integrated protein A affinity chromatography.