MALDI-TOF MS for microorganism identification: from pattern recognition towards marker based approaches
At a glance
- Project leader : Dr. Joël Pothier
- Project team : Dr. Fabio Rezzonico
- Project status : ongoing
- Funding partner : Internal
- Project partner : Mabritec AG
- Contact person : Joël Pothier
Description
In collaboration with Mabritec AG, we have developed a database
of an unprecedented nature, the PAPMID (putative assigned protein
masses for identification database). Based on over 12,000 sequences
of bacterial genomes, it contains the molecular masses of the
ribosomal proteins of these microorganisms and this allows for the
instant identification and subtyping of bacteria. In combination
with the spectra obtained by MALDI-TOF MS, this original database
is an important aid in the interpretation of phylogenetic
relationships between the investigated bacteria.
Not restricted to any one type of mass spectrometer, this database
will soon be available to customers via the Internet.
Publications
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Rothen, Julian; Sapugahawatte, Dulmini Nanayakkara; Li, Carmen; Lo, Norman; Vogel, Guido; Foucault, Frédéric; Pflüger, Valentin; Pothier, Joël; Blom, Jochen; Daubenberger, Claudia; Ip, Margaret,
2020.
Scientific Reports.
10(8788).
Available from: https://doi.org/10.1038/s41598-020-65707-5
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Fossou, Romain K.; Pothier, Joël F.; Zézé, Adolphe; Perret, Xavier,
2020.
International Journal of Systematic and Evolutionary Microbiology.
70(2), pp. 1421-1430.
Available from: https://doi.org/10.1099/ijsem.0.003931
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Rothen, Julian; Pothier, Joël F.; Foucault, Frédéric; Blom, Jochen; Nanayakkara, Dulmini; Li, Carmen; Ip, Margaret; Tanner, Marcel; Vogel, Guido; Pflüger, Valentin; Daubenberger, Claudia A.,
2019.
Frontiers in Microbiology.
10(471).
Available from: https://doi.org/10.3389/fmicb.2019.00471
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Normand, Philippe; Nouioui, Imen; Pujic, Petar; Fournier, Pascale; Dubost, Audrey; Schwob, Guillaume; Klenk, Hans-Peter; Nguyen, Agnès; Abrouk, Danis; Herrera-Belaroussi, Aude; Pothier, Joël F.; Pflüger, Valentin; Fernandez, Maria P.,
2018.
Frankia canadensis sp. nov., isolated from root nodules of Alnus incana subspecies rugosa.
International Journal of Systematic and Evolutionary Microbiology.
68, pp. 3001-3011.
Available from: https://doi.org/10.1099/ijsem.0.002939
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Rothen, Julian; Schindler, Tobias; Pothier, Joël F.; Younan, Mario; Certa, Ulrich; Daubenberger, Claudia; Pflüger, Valentin; Jores, Joerg,
2017.
Genome Announcements.
5(28).
Available from: https://doi.org/10.1128/genomeA.00525-17
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Ziegler, Dominik; Pothier, Joël F.; Ardley, Julie; Kouakou Fossou, Romain; Pflüger, Valentin; de Meyer, Sofie; Vogel, Guido; Tonolla, Mauro; Howieson, John; Reeve, Wayne; Perret, Xavier,
2015.
Ribosomal protein biomarkers provide root nodule bacterial identification by MALDI-TOF MS.
Applied Microbiology and Biotechnology.
99(13), pp. 5547-5562.
Available from: https://doi.org/10.1007/s00253-015-6515-3
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van der Wolf, Jan M.; Nijhuis, Els H.; Kowalewska, Malgorzata J.; Saddler, Gerry S.; Parkinson, Neil; Elphinstone, John G.; Pritchard, Leighton; Toth, Ian K.; Lojkowska, Ewa; Potrykus, Marta; Waleron, Malgorzata; de Vos, Paul; Cleenwerck, Ilse; Pirhonen, Minna; Garlant, Linda; Hélias, Valérie; Pothier, Joël F.; Pflüger, Valentin; Duffy, Brion; Tsror, Leah; Manulis, Shula,
2014.
International Journal of Systematic and Evolutionary Microbiology.
64, pp. 768-774.
Available from: https://doi.org/10.1099/ijs.0.052944-0