MALDI-TOF MS for microorganism identification: from pattern recognition towards marker based approaches
Description
In collaboration with Mabritec AG, we have developed a database of an unprecedented nature, the PAPMID (putative assigned protein masses for identification database). Based on over 12,000 sequences of bacterial genomes, it contains the molecular masses of the ribosomal proteins of these microorganisms and this allows for the instant identification and subtyping of bacteria. In combination with the spectra obtained by MALDI-TOF MS, this original database is an important aid in the interpretation of phylogenetic relationships between the investigated bacteria.
Not restricted to any one type of mass spectrometer, this database will soon be available to customers via the Internet.
Key Data
Projectlead
Project team
Project partners
Mabritec AG
Project status
ongoing, started 05/2013
Funding partner
Internal
Publications
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A simple, rapid typing method for Streptococcus agalactiae based on ribosomal subunit proteins by MALDI-TOF MS
2020 Rothen, Julian; Sapugahawatte, Dulmini Nanayakkara; Li, Carmen; Lo, Norman; Vogel, Guido; Foucault, Frédéric; Pflüger, Valentin; Pothier, Joël; Blom, Jochen; Daubenberger, Claudia; Ip, Margaret
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Bradyrhizobium ivorense sp. nov. as a potential local bioinoculant for Cajanus cajan cultures in Côte d’Ivoire
2020 Fossou, Romain K.; Pothier, Joël F.; Zézé, Adolphe; Perret, Xavier
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Subspecies typing of Streptococcus agalactiae based on ribosomal subunit protein mass variation by MALDI-TOF MS
2019 Rothen, Julian; Pothier, Joël F.; Foucault, Frédéric; Blom, Jochen; Nanayakkara, Dulmini; Li, Carmen; Ip, Margaret; Tanner, Marcel; Vogel, Guido; Pflüger, Valentin; Daubenberger, Claudia A.
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Frankia canadensis sp. nov., isolated from root nodules of Alnus incana subspecies rugosa
2018 Normand, Philippe; Nouioui, Imen; Pujic, Petar; Fournier, Pascale; Dubost, Audrey; Schwob, Guillaume; Klenk, Hans-Peter; Nguyen, Agnès; Abrouk, Danis; Herrera-Belaroussi, Aude; Pothier, Joël F.; Pflüger, Valentin; Fernandez, Maria P.
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Draft genome sequences of seven Streptococcus agalactiae strains isolated from Camelus dromedarius at the horn of Africa
2017 Rothen, Julian; Schindler, Tobias; Pothier, Joël F.; Younan, Mario; Certa, Ulrich; Daubenberger, Claudia; Pflüger, Valentin; Jores, Joerg
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Ribosomal protein biomarkers provide root nodule bacterial identification by MALDI-TOF MS
2015 Ziegler, Dominik; Pothier, Joël F.; Ardley, Julie; Kouakou Fossou, Romain; Pflüger, Valentin; de Meyer, Sofie; Vogel, Guido; Tonolla, Mauro; Howieson, John; Reeve, Wayne; Perret, Xavier
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Dickeya solani sp. nov., a pectinolytic plant-pathogenic bacterium isolated from potato (Solanum tuberosum)
2014 van der Wolf, Jan M.; Nijhuis, Els H.; Kowalewska, Malgorzata J.; Saddler, Gerry S.; Parkinson, Neil; Elphinstone, John G.; Pritchard, Leighton; Toth, Ian K.; Lojkowska, Ewa; Potrykus, Marta; Waleron, Malgorzata; de Vos, Paul; Cleenwerck, Ilse; Pirhonen, Minna; Garlant, Linda; Hélias, Valérie; Pothier, Joël F.; Pflüger, Valentin; Duffy, Brion; Tsror, Leah; Manulis, Shula
