Antibiotic resistances in Enterobacteria
Description
Atypical Enterobacteria are frequently misidentified in clinical diagnostics due to unsettled taxonomy and noisy reference databases. Despite of their environmental origin, they regularly appear in clinical samples and increasingly display multi-drug resistances (MDRs) that are acquired during adaptation to the nosocomial environment. Failure to provide a consistent identification inevitably results in the impossibility to recognize connections between seemingly independent clinical episodes and to implement proper surveillance and sanitary countermeasures. In this project, we aim (1) to accurately identify isolates collected during routine hospital environmental surveillance using mass spectrometry (MALDI-TOF MS), Multi-Locus Sequence Analysis (MLSA) and whole-genome sequencing (WGS); (2) to investigate the mechanisms by which these bacteria acquire antimicrobial resistance genes (ARGs); and (3) to identify genomic regions that can be used as targets to design efficient molecular tests for the detection of recurring species in clinical samples, to improve quality and speed of diagnosis.
Key Data
Projectlead
Deputy Projectlead
Project partners
Pontifícia Universidade Católica do Paraná; Central Public Health Laboratory
Project status
completed, 12/2020 - 11/2022
Funding partner
Leading House Lateinamerika
Publications
-
Genomic identification of two Phytobacter diazotrophicus isolates from a neonatal intensive care unit in Singapore
2023 Hon, Peiyun; Ko, Karrie K.K.; Zhong, Jonathan C.W.; De, Partha P.; Smits, Theo H. M.; Low, Jiaming; Vasoo, Shawn; Tsui, Clement K.M.
-
Correction for Hon et al., “Genomic Identification of Two Phytobacter diazotrophicus Isolates from a Neonatal Intensive Care Unit in Singapore”
2023 Hon, Peiyun; Ko, Karrie K. K.; Chia, Jonathan W. Z.; De, Partha P.; Smits, Theo H. M.; Low, Jiaming; Vasoo, Shawn; Tsui, Clement K. M.
-
Dissemination of NDM-producing bacteria in Southern Brazil
2023 Arend, Lavinia N.V.S.; Bergamo, Ricardo; Rocha, Fabiano B.; Bail, Larissa; Ito, Carmen; Baura, Valter A.; Balsanelli, Eduardo; Pothier, Joël F.; Rezzonico, Fabio; Pilonetto, Marcelo; Smits, Theo H.M.; Tuon, Felipe Francisco
-
Resolving taxonomic confusion : establishing the genus Phytobacter on the list of clinically relevant Enterobacteriaceae
2022 Smits, Theo H. M.; Arend, Lavinia N. V. S.; Cardew, Sofia; Tång-Hallbäck, Erika; Mira, Marcelo T.; Moore, Edward R. B.; Sampaio, Jorge L. M.; Rezzonico, Fabio; Pillonetto, Marcelo
-
Phytobacter diazotrophicus from intestine of Caenorhabditis elegans confers colonization-resistance against Bacillus nematocida using flagellin (FliC) as an inhibition factor
2022 Niu, Qiuhong; Liu, Suyao; Yin, Mingshen; Lei, Shengwei; Rezzonico, Fabio; Zhang, Lin
Einsiedlerstrasse 34
8820 Wädenswil