The objective is the development of a real-time pathogen screening system for a group of global relevant turf grass diseases using loop mediated isothermal amplification, LAMP technology. From a turf sample, DNA isolated and amplified using LAMP. Pathogen occurrence in the sample will be detected directly with the Genie® device by using a fluorescent dye. The detection tool will deliver a semi-quantitative disease occurrence diagnosis of the listed pathogens in one sample using basic molecular tools, in less than one hour.